Basic chemistry

chemists have been able to reconstruct an egg?

reconstruction of an egg?


Reconstruction of an egg by chemists :

Reconstruction of an egg by chemists is listed here briefly that how it will be possible. By applying a solution of urea to a hard egg white and centrifuging it, scientists were able to restore the protein called lysozyme. An amazing experience that, despite appearances, is far from useless.

When an egg is cooked, the proteins unfold, coagulate and the egg hardens. Can this seemingly irreversible process be reversed? Can a failed dish be repeated with the same ingredients ? Scientists from the University of Irvine (California) and Adelaide (Australia) publish in the journal ChemBioChem a protocol with which they managed to “deconstruct” an egg. Material needed: a urea solution and an innovative machine that centrifuge – not ideal for cooking .

But the experience is not so anecdotal as it seems. In fact, this process could revolutionize the industrial production of proteins, and lower the costs of many treatments, such as anti-cancer. Focus on this fast, efficient and inexpensive technique.

Lysozyme of egg white becomes functional again

In their article, the researchers worked on an egg white protein : lysozyme. The egg white was baked for 20 minutes at 90 ° C and then dissolved with urea to recover a liquid structure  . At this stage, the proteins are still unusable. Then comes the second step: a centrifugation with a powerful machine created at  South Australia’s Finders University .

The egg white rotates in a glass cylinder 10 mm by 16 cm at 5,000 rpm with a 45 ° angle. And then miracle: lysozyme has regained its activity. The method has also been used with recombinant caveolin and a larger protein, protein kinase A (or  cAMP-dependent protein kinase A : PKA).

This is a victory for the researchers who fought long with lab-produced proteins that did not take the desired form. This discovery could bring a lot to the pharmaceutical industry in particular.

A revolution for the production of recombinant proteins

This method could transform protein production for research and industry. Indeed, the production of recombinant proteins is less expensive in models such as yeast or E. coli bacteria  . But often the proteins produced do not have the right configuration, form aggregates and do not fold correctly. This is why other cellular models of recombinant protein production , such as mammalian cells or insect cells – much more expensive models are used. For example, the pharmaceutical industry must produce antibodies against cancer in a model of hamster ovary cells, in order to obtain a good folding of the proteins.

There are methods to recover misfolded material, but these are expensive and tedious. The equivalent of dialysis at a molecular level requires several days and liters of fluid to obtain mg of protein. The new process described here is much faster and cheaper. It could therefore reduce the production costs of certain drugs.

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